Courtney Howard Represents WKU at HHMI Science Education Alliance Symposium

Courtney Howard (Hardin, ‘11) recently returned from the 2nd Annual Science Education Alliance Symposium at the Janelia Farm campus of the Howard Hughes Medical Institute (HHMI) in Ashburn, Virginia. There, she presented her research work and the research work of the inaugural class of WKU’s HHMI’s Science Education Alliance National Genomics Research Initiative course.

After a full-year experience in the nationally-sponsored research program that is overseen at WKU by Drs. Rodney King and Claire Rinehart from WKU’s Department of Biology, Howard was asked by her instructors to represent WKU, since it was her virus that was completely sequenced and annotated.

Last fall semester, Howard isolated a novel bacterial virus from a soil sample she collected from her home in Radcliff, KY, naming the virus “Backyardigan” after a television show she used to watch as a kid.

Dr. King described the research work Howard undertook during the fall semester after this initial collection. “She grew large numbers of the virus in the lab and examined its structure using an electron microscope. She then isolated the DNA from the virus and characterized it by cutting it with special enzymes called restriction endonucleases. The pattern of DNA fragments generated from this treatment was then examined and compared to other viruses isolated by her classmates and by other NGRI participants located throughout the country. Courtney’s viral DNA preparation passed rigorous quality control tests and it was chosen to have its DNA sequence determined,” he said.

During the spring semester, the WKU Genome Discovery class identified all the genes contained in the DNA sequence of the virus.

Howard is already looking forward to what may happen next. “I had a wonderful experience at the conference. It renewed my interest in research. As a matter of fact Dr. Rinehart and I are currently discussing ways to extend my phage research. Listening to the presentations opened our eyes to other possibilities and things we can do to learn more about my phage,” she said.

The slides from Howard’s talk, titled 007: A Lysin to Kill in my Backyardigan, are available at the following weblink: http://www.hhmi.org/seawiki/download/attachments/11993294/23_WEKU.pdf

For more information, contact Derick Strode at (270) 745-6565.

Courtney Howard, HHMI, National Genomics Research Initiative, virus, WKU